Journal: Advanced Science

Article Title: Distinct CTC Specific RNA Profile Enables NSCLC Early Detection and Dynamic Monitoring of Advanced NSCLC

doi: 10.1002/advs.202417849

Figure Lengend Snippet: NSCLC characteristic mRNA selection and optimization of CTC purification. A) Illustration of NSCLC specific mRNA selection. B) Expression levels of 6 genes in PBMCs and four NSCLC cell lines. C) Expression levels of 6 genes in PBMCs isolated from different patients. D) Illustration of CTC purification using artificial samples. Optimization of concentrations of E) anti‐EpCAM, F) anti‐EGFR, and G) anti‐N‐cadherin for CTC capture. H) A respective SEM image of an isolated cancer cell with mounts of MNPs on its surface. I) The relative expression levels of EpCAM, EGFR, and N‐cadherin on these four cell lines were analyzed by flow cytometry. J) Capture performance of the antibody cocktail‐modified MNPs for the four cancer cell lines.

Article Snippet: Multi‐antibody‐modified MNPs were prepared as follows: 0.1 mg of streptavidin‐modified beads (Beaver) were incubated for 30 min with a mixture of antibodies, including biotinylated anti‐human EpCAM (R&D Systems, polyclonal goat IgG, 200 ng/200 μL), biotinylated anti‐human EGFR (R&D Systems, polyclonal goat IgG, 200 ng/200 μL), and biotinylated anti‐human N‐cadherin (Proteintech, polyclonal rabbit IgG, 200 ng/200 μL).

Techniques: Selection, Purification, Expressing, Isolation, Flow Cytometry, Modification